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ras activation elisa kit  (Millipore)


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    Millipore ras activation elisa kit
    Rab25 inactivates EGFR. Cancer cells were transfected with the indicated vectors for 24 h. (A) The cell lysates were analyzed by immunoblotting with the indicated antibodies. (B) The p-EGFR expression was visualized using immunofluorescence. Original magnification, ×400; scale bar, 20 µm. (C) <t>Ras</t> activity was analyzed using the Ras-GTP pull down assay. Total protein expressions were analyzed by immunoblotting with the indicated antibodies. (D) Ras activity was analyzed using the Ras activation <t>ELISA</t> kit (***P < 0.001 vs. control vector). (E) HCT-116 cells were transfected with the indicated vectors for 24 h. Invasion was analyzed by utilizing a 48-well chemotaxis chamber (**P < 0.01 vs. control vector, ***P < 0.001 vs. control vector and ### P < 0.001 vs. Rab25 overexpression). Representative results of at least three independent experiments with similar results. Rab25, Ras-related protein 25; p-, phosphorylated; IP, immunoprecipitation; WB, western blotting.
    Ras Activation Elisa Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ras+activation+elisa+kit/pmc10777460-105-29-36?v=Millipore
    Average 90 stars, based on 1 article reviews
    ras activation elisa kit - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Rab25 suppresses colon cancer cell invasion through upregulating claudin‑7 expression"

    Article Title: Rab25 suppresses colon cancer cell invasion through upregulating claudin‑7 expression

    Journal: Oncology Reports

    doi: 10.3892/or.2023.8685

    Rab25 inactivates EGFR. Cancer cells were transfected with the indicated vectors for 24 h. (A) The cell lysates were analyzed by immunoblotting with the indicated antibodies. (B) The p-EGFR expression was visualized using immunofluorescence. Original magnification, ×400; scale bar, 20 µm. (C) Ras activity was analyzed using the Ras-GTP pull down assay. Total protein expressions were analyzed by immunoblotting with the indicated antibodies. (D) Ras activity was analyzed using the Ras activation ELISA kit (***P < 0.001 vs. control vector). (E) HCT-116 cells were transfected with the indicated vectors for 24 h. Invasion was analyzed by utilizing a 48-well chemotaxis chamber (**P < 0.01 vs. control vector, ***P < 0.001 vs. control vector and ### P < 0.001 vs. Rab25 overexpression). Representative results of at least three independent experiments with similar results. Rab25, Ras-related protein 25; p-, phosphorylated; IP, immunoprecipitation; WB, western blotting.
    Figure Legend Snippet: Rab25 inactivates EGFR. Cancer cells were transfected with the indicated vectors for 24 h. (A) The cell lysates were analyzed by immunoblotting with the indicated antibodies. (B) The p-EGFR expression was visualized using immunofluorescence. Original magnification, ×400; scale bar, 20 µm. (C) Ras activity was analyzed using the Ras-GTP pull down assay. Total protein expressions were analyzed by immunoblotting with the indicated antibodies. (D) Ras activity was analyzed using the Ras activation ELISA kit (***P < 0.001 vs. control vector). (E) HCT-116 cells were transfected with the indicated vectors for 24 h. Invasion was analyzed by utilizing a 48-well chemotaxis chamber (**P < 0.01 vs. control vector, ***P < 0.001 vs. control vector and ### P < 0.001 vs. Rab25 overexpression). Representative results of at least three independent experiments with similar results. Rab25, Ras-related protein 25; p-, phosphorylated; IP, immunoprecipitation; WB, western blotting.

    Techniques Used: Transfection, Western Blot, Expressing, Immunofluorescence, Activity Assay, Pull Down Assay, Activation Assay, Enzyme-linked Immunosorbent Assay, Control, Plasmid Preparation, Chemotaxis Assay, Over Expression, Immunoprecipitation



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    ras activation elisa kit  (Millipore)
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    Millipore ras activation elisa kit
    Rab25 inactivates EGFR. Cancer cells were transfected with the indicated vectors for 24 h. (A) The cell lysates were analyzed by immunoblotting with the indicated antibodies. (B) The p-EGFR expression was visualized using immunofluorescence. Original magnification, ×400; scale bar, 20 µm. (C) <t>Ras</t> activity was analyzed using the Ras-GTP pull down assay. Total protein expressions were analyzed by immunoblotting with the indicated antibodies. (D) Ras activity was analyzed using the Ras activation <t>ELISA</t> kit (***P < 0.001 vs. control vector). (E) HCT-116 cells were transfected with the indicated vectors for 24 h. Invasion was analyzed by utilizing a 48-well chemotaxis chamber (**P < 0.01 vs. control vector, ***P < 0.001 vs. control vector and ### P < 0.001 vs. Rab25 overexpression). Representative results of at least three independent experiments with similar results. Rab25, Ras-related protein 25; p-, phosphorylated; IP, immunoprecipitation; WB, western blotting.
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    ras activation assay kit for elisa  (Millipore)
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    Millipore ras activation assay kit for elisa
    Rab25 inactivates EGFR. Cancer cells were transfected with the indicated vectors for 24 h. (A) The cell lysates were analyzed by immunoblotting with the indicated antibodies. (B) The p-EGFR expression was visualized using immunofluorescence. Original magnification, ×400; scale bar, 20 µm. (C) <t>Ras</t> activity was analyzed using the Ras-GTP pull down assay. Total protein expressions were analyzed by immunoblotting with the indicated antibodies. (D) Ras activity was analyzed using the Ras activation <t>ELISA</t> kit (***P < 0.001 vs. control vector). (E) HCT-116 cells were transfected with the indicated vectors for 24 h. Invasion was analyzed by utilizing a 48-well chemotaxis chamber (**P < 0.01 vs. control vector, ***P < 0.001 vs. control vector and ### P < 0.001 vs. Rab25 overexpression). Representative results of at least three independent experiments with similar results. Rab25, Ras-related protein 25; p-, phosphorylated; IP, immunoprecipitation; WB, western blotting.
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    Image Search Results


    Rab25 inactivates EGFR. Cancer cells were transfected with the indicated vectors for 24 h. (A) The cell lysates were analyzed by immunoblotting with the indicated antibodies. (B) The p-EGFR expression was visualized using immunofluorescence. Original magnification, ×400; scale bar, 20 µm. (C) Ras activity was analyzed using the Ras-GTP pull down assay. Total protein expressions were analyzed by immunoblotting with the indicated antibodies. (D) Ras activity was analyzed using the Ras activation ELISA kit (***P < 0.001 vs. control vector). (E) HCT-116 cells were transfected with the indicated vectors for 24 h. Invasion was analyzed by utilizing a 48-well chemotaxis chamber (**P < 0.01 vs. control vector, ***P < 0.001 vs. control vector and ### P < 0.001 vs. Rab25 overexpression). Representative results of at least three independent experiments with similar results. Rab25, Ras-related protein 25; p-, phosphorylated; IP, immunoprecipitation; WB, western blotting.

    Journal: Oncology Reports

    Article Title: Rab25 suppresses colon cancer cell invasion through upregulating claudin‑7 expression

    doi: 10.3892/or.2023.8685

    Figure Lengend Snippet: Rab25 inactivates EGFR. Cancer cells were transfected with the indicated vectors for 24 h. (A) The cell lysates were analyzed by immunoblotting with the indicated antibodies. (B) The p-EGFR expression was visualized using immunofluorescence. Original magnification, ×400; scale bar, 20 µm. (C) Ras activity was analyzed using the Ras-GTP pull down assay. Total protein expressions were analyzed by immunoblotting with the indicated antibodies. (D) Ras activity was analyzed using the Ras activation ELISA kit (***P < 0.001 vs. control vector). (E) HCT-116 cells were transfected with the indicated vectors for 24 h. Invasion was analyzed by utilizing a 48-well chemotaxis chamber (**P < 0.01 vs. control vector, ***P < 0.001 vs. control vector and ### P < 0.001 vs. Rab25 overexpression). Representative results of at least three independent experiments with similar results. Rab25, Ras-related protein 25; p-, phosphorylated; IP, immunoprecipitation; WB, western blotting.

    Article Snippet: The cells were cultured to ~80% confluency in a 35 mm dish and were transfected for 24 h. The supernatants were removed and RAS activation was determined using a RAS activation ELISA kit (cat. no. 17-497; Millipore Sigma) according to the manufacturer's instructions.

    Techniques: Transfection, Western Blot, Expressing, Immunofluorescence, Activity Assay, Pull Down Assay, Activation Assay, Enzyme-linked Immunosorbent Assay, Control, Plasmid Preparation, Chemotaxis Assay, Over Expression, Immunoprecipitation